About 5 years ago Elena called me and asked, without much introduction: – Can you characterize the genetics structure of Baikal zooplankton population? – What, right now? – said I. – No, in the future, if we get funded? – I can try, said I. Lots of time elapsed. We did get funded. The plans to characterize the genetic differentiation of Baikal Epischura changed from microsats (oh so 20th century) to RADseq to, finally, pooled RNAseq, which is what we ended up doing. So. Is there or is there no genetic differentiation among samples of Epischura from different parts of Baikal? On Wednesday Larry will have his MS defense and, with his permission, I am posting some of our results here.
Fig. 1. Principle component analysis based on minor allele frequency of 2000 randomly selected SNPs. Individual points are pooled RNAseq biological replicates, 3 per sample. Blue – South Baikal (“St.1”), red – Central Baikal, yellow – North Baikal, green – Maloe More.
Fig. 2. Actual (green) and expected based on random assignment of alleles to populations distribution of Fst values in 4 samples shown on Fig.1.
We expect to add 2 more samples from each region plus separate top 50 and 250-100 m depth layers at Station 1. Hopefully before summer.